Primer

Part:BBa_K5103006

Designed by: Nikoo Mansourian   Group: iGEM24_Guelph   (2024-09-22)


Forward primer introducing a HindIII cut site to the 5' end of Cry8Da

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Profile

Name: Forward primer introducing a HindIII cut site to the 5' end of Cry8Da
Base Pairs: 35 bp
Origin: NA
Properties: Encodes a HindIII restriction site, compatible with part: Part:BBa_K5103000

Usage and Biology

This part is a forward primer designed to introduce a HindIII restriction site at the 5’ end of the Cry8Da gene. This primer allows for the directional cloning of Cry8Da into expression plasmids that utilize the HindIII site for gene insertion. This primer is intended for use in systems where HindIII cut sites are necessary for cloning Cry8Da into plasmid backbones such as pYES2 for yeast.

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Categories
Parameters
None